Figure 9
From: Highly Stable and Sensitive Fluorescent Probes (LysoProbes) for Lysosomal Labeling and Tracking

Visualization of lysosomal alterations using LysoProbe II in RBE cells undergoing apoptosis:
(A–C): cells treated with CQ in standard serum for 16 h: (A) CQ (50 μM); (B) CQ (100 μM); (C) CQ (200 μM); (D) CQ (300 μM); (E): after treatment with CQ (50 μM), in the absence of standard serum, for 16 h. Cells were imaged on an inverted laser scanning fluorescent microscope (Olympus) using a 60 × oil immersion objective lens.