Table 1 HemB enzyme activity in different recombinant strains

From: Optimization of the heme biosynthesis pathway for the production of 5-aminolevulinic acid in Escherichia coli

Recombinants

HemB activity (U mg−1)a

Protoporphyrin IX (mg l−1)b

E. coli LA

6.97 ± 0.26

-

E. coli LAF

3.34 ± 0.05

-

E. coli LAG

22.21 ± 0.42

-

E. coli LAB

52.04 ± 1.47

-

E. coli LAB

51.78 ± 1.50

5.0

E. coli LAB

51.70 ± 1.53

10.0

  1. aData are the means ± standard deviation (SD) from three parallel experiments.
  2. bIntermediate protoporphyrin IX was added into the reaction system to study the effect on HemB activity.
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