Figure 5
LF-MF exposure and protein aggregation.
(A, B) Extracts from spinal cord tissues from female SOD1G85R (A) and SOD1G93A (B) mutant mice at endstage were separated by centrifugation. The soluble and the pellet fraction were analyzed by Western blot and polyubiquitinated proteins, HSP25 and SOD1 were detected by specific antibodies. Non-exposed non-transgenic littermates (ntg. n.ex.) and non-exposed SOD1WT mice served as controls. The whole panels of the blots are displayed in Supplemental Fig. 13 and Supplemental Fig. 14 for SOD1G85R and SOD1G93A, respectively. (C–E) For the densitometric analysis of Western blots the results from female and male SOD1G85R mice (see Supplemental Fig. 5) were pooled. Results from exposed animals were normalized to sham exposed animals (mean +/−SEM; n = 6; t-test, n.s. not significant).
