Figure 6
Oxidative markers are not modulated by LF-MF.
(A, B) Total extracts from spinal cords of SOD1G93A (A) and SOD1G85R mice (B) were separated on PAGE gels without β-mercaptoethanol in the loading buffer. After Western blotting, SOD1 was detected with specific antibodies. Densitometric analysis is shown in lower panels. Data from SOD1G93A and SOD1G85R mice were compared to non-exposed mice overexpressing human wild-type SOD1 (wt n.ex.) or non-transgenic littermates (ntg. n.ex.), respectively. (mean +/-SEM; n = 6; t-test, n.s. not significant). (C, D) Carbonylated proteins in spinal cord extracts from female SOD1G93A and SOD1G85R mice at endstage were derivatized by dinitrophenylhydrazin (DNP) and detected by Western blot with specific antibodies directed against DNP. Tubulin served as a loading control and was developed on the same membrane. Results from SOD1G93A and SOD1G85R mice were normalized to non-exposed mice overexpressing human wild-type SOD1 (wt n.ex.) or non-transgenic littermates (ntg. n.ex.), respectively (mean +/−SEM, n = 8, t-test; * p<0.05).
