Figure 3
Dose–dependent response of non-thermal plasma and ROS-generating systems on the cancer and normal cells.
Viability of cancer and normal cells treated with (a) plasma, (b) plasma plus scavengers, (c) H2O2, (d) X/XO [xanthine/xanthine oxidase], for O2− (e) CPA [CuSO4, phenanthroline and ascorbic acid] for HO· radicals. (f) Immunofluorescence assays using phalloidin rhodamine (5 units/ml, Invitrogen) were performed to visualize the cytoskeleton (F-actin); DAPI was used to label cell nucleus in plasma and plasma plus scavengers treated T98G cells. Each figure has scale bar of 10 μm. Results are expressed as the percentage of living cells compared to control conditions as the mean ± SD (n = 3). Student's t-test was performed to controls in plasma, H2O2, X/XO (O2−), CPA (HO·), whereas in (a) and (b) plasma and plasma plus scavenger-treated groups were compared to untreated and only plasma-treated group, respectively (* p < 0.05, § p < 0.01, # p < 0.001).
