Figure 6
Analysis of apoptotic indicators of plasma and plasma plus scavengers treated cancer cells.
(a) Flow cytometric band shift plot of mitochondrial membrane potential in T98G cells, where cccp was used as a positive control. (b) A bar graph of calculated JC-1 red fluorescence percent intensity changes of T98G cells (c) Flow cytometric band shift plot of mitochondrial membrane potential in A549 cells. (d) A bar graph of JC-1 red fluorescence calculated percent intensity changes of A549 cells. Apoptotic cell death was assessed using annexin V-FITC/PI staining and flow cytometry. Stained cells can be discriminated cells into four groups, i.e., the viable (annexin V− PI−), early apoptosis (annexin V+ PI−), late apoptosis (annexinV+ PI+) and necrotic (annexin V− PI+) groups. (e) A plot of T98G cells apoptotic population treated by plasma and plasma plus scavengers. (f) A plot of A549 cells apoptotic population treated by plasma and plasma plus scavengers. (g) Contour plot of T98G cells population treated with plasma and plasma plus scavengers. Results are expressed as the mean ± SD (n = 3). Student's t-test was performed to control, whereas in plasma plus scavenger-treated group was compared to only plasma-treated group (* p < 0.05, § p < 0.01, # p < 0.001).
