Figure 1
From: SNORD76, a box C/D snoRNA, acts as a tumor suppressor in glioblastoma
SNORD76, rather than its host gene (GAS5), is elevated after HOTAIR knockdown in glioma cell lines.
Detection of HOTAIR (A), SNORD76 (B) and GAS5 (C) expression by qRT-PCR in glioma cell lines. Numbers above solid bars are the relative expression when compared to U87-MG. (D) Quantitative PCR validation of HOTAIR knockdown efficacy in LN229 and U87-MG glioma cell lines. (E) and (F) Expression of SNORD76 and GAS5 as determined by qPCR after HOTAIR siRNA treatment. The expression of HOTAIR (G), SNORD76 (H) and GAS5 (I) was measured by qPCR after infection with lentivirus expressing the full length of HOTAIR. All data represent means ± s.e.m. of three experiments performed in triplicate. *P < 0.05, **P < 0.01 (Student's t test), ns, no significant difference (P ≥ 0.05) relative to the control group.
