Figure 3
From: WNT-1 inducible signaling pathway protein-1 enhances growth and tumorigenesis in human breast cancer
Effect of ectopic WISP1 overexpression on cell cycle distribution of MCF-7 cells.
(A). The cell cycle distribution of MCF7-DNA and MCF7-WISP1-1 cells was analyzed by flow cytometry after 48 hours incubation. The data shown in each bar chart represent the mean percentage ± SE (n = 5) of cells in each phase of the cell cycle. (B). The expression (Cropped) and quantitative analysis of cyclins, p21, p27, WISP1 and BTG2 in MCF7-DNA and MCF7-WISP1-1 cells were determined by immunoblotting assays. Data are presented as fold-induction of target genes after WISP1-overexpressed. (*P < 0.01).
