Figure 7
From: WNT-1 inducible signaling pathway protein-1 enhances growth and tumorigenesis in human breast cancer
Evaluation of the effect of WISP1 overexpression on MCF-7 cell tumorigenesis and progression in vivo.
MCF7-DNA (A) and MCF7-WISP1-1 (B) cells (5 × 106) were equally mixed with matrigel and then injected subcutaneously into the back area of each nude mouse. The tumor volumes (mm3) in the MCF7-DNA group (•) and the MCF7-WISP1-1 group (○) were measured regularly (C). Data are presented as the mean ± SE. Scale bar, 10 mm. (D) Whole-cell lysates of randomly selected tumor samples from MCF7-DNA (A) and MCF7-WISP1-1 groups were subjected to RT-qPCR. Data are presented as mean fold induction of the WISP1 mRNA (± SE; n = 3) relative to the mock-transfected xenograft groups. (+P < 0.05, *P < 0.01).
