Figure 8

NDRG1-overexpression mitigates the effect of WISP1 in breast cancer cell proliferation and invasion.

NDRG1 expression in transiently NDRG1-transfected MCF7 (MCF7-NDRG1) (A) and MDA-MB-231 (MDA-NDRG1) (D) cells was determined by western blot and RT-qPCR. Recombinant WISP1 was used to treat MCF-7 and MCF7-NDRG1 cells (B) or MDA-MB-231 and MDA-NDRG1cells (E) with the indicated concentrations for 2 days. Cell proliferation was measured using the CyQUANT cell proliferation assay kit. The cell invasion ability of MCF-7 and MCF7-NDRG1 cells (C) or MDA-MB-231and MDA-NDRG1 cells (F) after 48 hours incubation with 1000 ng/ml of recombinant WISP was measured using the matrigel-invasion assay. Each point of the curve represents the mean percentage ± SE (n = 6) in relation to that of control-solvent cells. (⁺P < 0.05, *P < 0.01).