Figure 4
Anti-fibrotic effects of Ad-MSCs on irradiated lung.
(A) IHC-staining for α-SMA. Left rank: Magnification at 200×. Scale bar, 100 μm. Right rank: Magnification at 1000×. Scale bar, 20 μm (B) IHC-staining for TGF-β1. Left rank: Magnification at 200×. Scale bar, 100 μm. Right rank: Magnification at 1000×. Scale bar, 20 μm. (C) Concentrations of cytokines in serum. Data represent the mean ± S.D. of six independent samples. This experiment was performed only once. For statistical analysis, the data of six samples were calculated for the value of mean ± S.D.One-way ANOVA analysis was used for comparing the differences of data among groups. (D) Concentrations of cytokines in bronchoalveolar lavage fluid (BALF). The data of three samples from each group were collected in each independent experiment. The experiment was repeated for twice. For statistical analysis, the data of six samples in all two independent experiments were calculated for the value of mean ± S.D. One-way ANOVA analysis was used for comparing the differences of data among groups.*P ≤ 0.05 and **P ≤ 0.01 versus the PBS and Fibroblast groups. $P ≤ 0.05 and $$P ≤ 0.01 versus the PBS and Fibroblast groups. It should be noted that the assays used to test for HGF, PGE2, TGF-β1 and TNF-α were for rat tissues, not human tissues.(E) Expression of HGF in irradiated lung. (F) Expression of mPGES in irradiated lung. All primers of above genes are designed for rat species. The expression levels of HGF and mPGES were tested using quantitative real-time PCR. rActin was set as the internal control for determining ΔCT values. Fold increases in expression were normalized to normal group by determining 2−ΔΔCTvalues. The data of nine samples from each group were collected in only one independent experiment. For statistical analysis, the data of nine samples were calculated for the value of mean ± S.D. One-way ANOVA analysis was used for comparing the differences of data among groups. *P ≤ 0.05 and **P ≤ 0.01 (significant high) versus the PBS and Fibroblast groups. $P ≤ 0.05 and $$P ≤ 0.01 (significant low) versus PBS and Fibroblast groups. ns: no statistical significance.
