Figure 4 | Scientific Reports

Figure 4

From: In1-ghrelin splicing variant is overexpressed in pituitary adenomas and increases their aggressive features

Figure 4

Ghrelin and In2-ghrelin variant in AtT20 cell line.

(A) Expression profile of the ghrelin system (Ghrelin, In2-ghrelin variant, Mboat4 enzyme and Ghsr) in AtT-20 cell line obtained by qPCR. Data represent absolute mRNA copy number adjusted by a normalization factor (NF) calculated from the expression levels of three control genes (Actb, Ppia and Hprt); (B) ACTH secretion in response to 4 h ghrelin and In2-ghrelin variant treatment; (C) Cell viability in response to ghrelin and In2; IGF-1 and Paclitaxel were used as positive and negative controls, respectively. Values represent the mean ± S.E.M of 3–5 independent experiments. Asterisks (* p < 0.05, *** p < 0.001) indicate data that significantly differ from controls.

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