Figure 5
Cell viability and apoptosis in response to In1-ghrelin and/or ghrelin in tumoral pituitaries.
Effect of 24-, 48- and/or 72-h treatment of acylated ghrelin (100 nM) and/or acylated In1-ghrelin derived peptides (In1-19 and In1-40; 100 nM) on cell viability in human GH-omas (A; n = 5–17), ACTH-omas (B; n = 2–8), NFPAs (C; n = 3–15) and PRL-omas (D; n = 2–3) primary cell cultures, determined by alamar-blue reduction. Effect of 12–14 h treatment with acylated ghrelin and In1-19 (100 nM) on apoptotic rate in human GH-omas (E; n = 3) and ACTH-oma (F; n = 1), determined by Annexin V/Propidium Iodide staining. Data are expressed as percent of vehicle-treated controls (set at 100%) within experiment. Values represent the mean ± S.E.M. Asterisks (* p < 0.05, ** p < 0.01; *** p < 0.001) indicate significant differences between vehicle- and peptide-treatments.
