Figure 3
Tamoxifen resistant cell lines had a low level of miR-320a and a high level of c-Myc, Cyclin D1, ARPP-19 and ERRγ.
(a). Cell viability analysis of MCF-7-TamR cells as well as its parental cells treated with different doses of tamoxifen. (b). Real-time PCR analysis of miR-320a expression in TamR cells compared with parental cells. (c) and (d). Western blot analysis of the expression of c-Myc, Cyclin D1, ARPP-19 and ERRγ in MCF-7-TamR cells as well as parental cells. (e). Real-time PCR analysis of ARPP-19 and ERRγ mRNA expression in TamR cells compared with parental cells. Results are presented as an average of at least three replicates. ***P < 0.001.
