Figure 4
Re-expression of miR-320a or knock-down of its targets in TamR cells sensitizes these cells to tamoxifen.
(a) and (b). Cell viability (a) and soft agar colony formation (b) analysis of TamR cells transfected with miR-320a mimics. (c). The expression of ARPP-19, ERRγ, c-Myc and Cyclin D1 was examined by Western blot after transfected with indicated siRNA or siRNA and plasmids for 48 h. ARPP-19, c-Myc and Cyclin D1 were labeled with FLAG tag and ERRγ was labeled with GFP tag. d and e. Cell viability (d) and soft agar colony formation (e) analysis of TamR cells transfected with si-ARPP-19, -ERRγ, -c-Myc and -Cyclin D1. Results are shown as the average and s.d. of at least three biological and two technical replicates each,*P < 0.05, **P < 0.01, ***P < 0.001.
