Figure 3
Fluorescent microarray scanner images of NAPPA subarrays for different sealing methods.
Enlargements of fluorescent microarray scanner images of nucleic acid programmable protein arrays (NAPPA) subarrays processed using five different methods of sealing microreactors. Bright spots are fluorescently labeled proteins expressed in situ from plasmid DNA using in vitro coupled transcription translation (IVTT) reagent. Proteins are captured at each spot with a printing mixture containing capture antibodies. Spots adjacent to the bright spots just have the printing mixture without DNA. Therefore signal in adjacent spots indicates diffusion from the spots containing DNA. The five subarrays correspond to the following five sealing methods: (A) viscous liquid, (B) no sealing i.e flat glass, (C) silicone rubber, (D) gas, (E) hydraulic oil. Brightness and contrast of the right third of subarray (A) is separately adjusted to show actual printing density and locations of the adjacent spots. Brightness and contrast of the image as a whole is adjusted to help visualize diffusion to compare the different methods.
