Figure 6
From: βA3/A1-crystallin is a critical mediator of STAT3 signaling in optic nerve astrocytes
Active STAT3 binding site in the Cryba1 promoter.
A 2.5 fold induction of luciferase activity was observed in WT mouse astrocytes transfected with the pGL3-Cryba1 promoter vector and treated with IL-6, compared to astrocytes treated with the vehicle control. Treatment with Stattic + IL-6 resulted in ~50% reduction in luciferase activity. In contrast, when WT astrocytes were transfected with the pGL3-Cryba1ΔSTAT3 vector, eliminating the STAT3 binding sites, luciferase activity was not affected by treatment with IL-6 or Stattic. Error bars indicate s.d.; *P<0.05.
