Figure 5
From: Structure of human dipeptidyl peptidase 10 (DPPY): a modulator of neuronal Kv4 channels
DPP10 glycosylation.
(A) The glycosylated Asn-257 is placed adjacent to the β-propeller loop and might stabilize it. Both subunits are represented as ribbons, the β-propeller loops as cartoons and Asn-257 is shown as sticks. Subunit A is shown in light grey and green, while subunit B is shown in dark grey and magenta. (B) Interactions between the carbohydrate and the residues in the subunit A. Ser-229 makes a hydrogen bond with O5 (possibly O6 in subunit B) while Leu-222, Met-255, Leu-260, Tyr-295 and Lys-293 contribute with hydrophobic interactions. Glycan 2Fo-Fc map contoured at 1σ is shown in blue. (C) The glycosylation at Asn-342 might indirectly stabilize the β-propeller loop, since it is positioned relatively close to this region. The protein is shown as light grey cartoon and the β-propeller loop is shown as green cartoon. (D) N-acetylglucosamine bound to Asn-748 in the subunit B (shown as dark grey cartoon) possibly interacts through a hydrogen bond with Lys-775 of subunit A (shown as light grey cartoon). The carbohydrates are shown as yellow sticks and the hydrogen bond as red dashes.
