Figure 7
From: Comparison between single-molecule and X-ray crystallography data on yeast F1-ATPase
Six dwells at saturating ATP.
(A) Six dwells are visible at saturating ATP for mutant βR408I. (B–E) Examples showing occasional dwells between major steps for (B) βR408G, (C) αF405S, (D) wild type and (E) γI270T. Note that while (A) is typical of data for βR408I, (substeps were detected by eye in only 1/21, 6/160, 3/45 and 5/360 high quality data traces for (B) βR408G, (C) αF405S, (D) wild type and (E) γI270T respectively. All traces shown were recorded at 10 kHz and 3 mM ATP, except (C) which was at 1 mM ATP. The expected ATP-dependent dwell is ~1/6 frame at 3 mM ATP and would not be resolved. Scale bar marks 40 nm.
