Table 1 Dwell state kinetics

From: Comparison between single-molecule and X-ray crystallography data on yeast F1-ATPase

Form

  Michaelis Menten fits

  Dwell analysis

 

vmax (rev s−1)

Km (μM)

kATP (s−1 μM−1)

n

kATP (s−1 μM−1)

k2 (ms)−1

k3 (ms)−1

n

Wild type

586 ± 24

62 ± 9

28.3 ± 3.6

2177

18 ± 6

2.5 ± 1.2

4.3 ± 2.3

18, 28

γG278D

582 ± 137

63 ± 33

27.6 ± 10.2

89

26 ± 2

2.6 ± 0.6

5.0 ± 1.7

4, 5

βV279F

144 ± 13

24 ± 5

17.9 ± 2.8

537

15 ± 2

0.36 ± 0.08

2.3 ± 2.7

4, 8

αN67I

248 ± 47

41 ± 12

18.0 ± 2.6

432

14 ± 3

0.46 ± 0.09

2.3 ± 0.7

7, 3

βR408G

80 ± 11

10.0 ± 2.6

24.1 ± 4.0

439

19 ± 5

0.21 ± 0.08

1.3 ± 0.5

10, 17

αF405S

336 ± 40

49 ± 11

20.4 ± 3.2

1044

16 ± 6

0.8 ± 0.6

2.0 ± 0.9

62, 40

γI270T

377 ± 92

44 ± 15

25.7 ± 4.4

2190

22 ± 7

0.8 ± 0.5

2.5 ± 2.0

109, 27

βR408I

69 ± 13

7.9 ± 2.5

26.4 ± 4.7

6519

23 ± 6

0.20 ± 0.07

3.3 ± 2.3

384, 51

Bacillus PS333

129 ± 22

15 ± 5

25.8

     

E. coli 6

449 ± 39

21.1 ± 1.0

43 ± 4

     
  1. The results of the analysis from the Michaelis-Menten fits and the dwell time histogram analysis for wild type and the seven mutants screened. All values are quoted with 95% confidence intervals, with the exception of Bacillus PS3 and E. coli, where the values were sourced from previous studies as indicated in the table. The errors in these studies were typically quoted as standard deviations; for comparison to the 95% CIs listed here they have been multiplied by 1.96 for data where the number of degrees of freedom were unknown, or by the appropriate t-statistic where the number of degrees of freedom was known (2.45 to 1.96 for df = 6 to ∞). In some cases the error could not be calculated from the available data and has been left blank. The number of traces used is denoted by n. For the dwell analysis, the first value of n corresponds to the number of traces used to calculate kATP and the second value for the number of traces used to calculate k2 and k3.
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