Table 5 Post-integration removal of the flanking piggyBac transposons in TS3 individuals obtained by crossing TS2-RgG2 moths with wild-type 871 moths

From: An efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori

Group

TS2-RgG2 fertile moths

G3 broods

G3 broods containing TS3 larvae with different fluorescence phenotype (%)

Total (%)a

Rg-positive broods

gG-positive broods

g-positive broods

1#

54

48

0

2 (4.17)

0

2 (4.17)

2#

41

37

7 (18.92)

18 (48.65)

6 (16.22)

23 (62.16)

3#

51

45

3 (6.67)

6 (13.33)

1 (2.22)

7 (15.56)

  1. Group 1#, 2# and 3# represent the individuals of G2 broods without HST, HST in the embryonic stage and HST in the larval stage, respectively (as described in Supplementary Figure S4B and Supplementary Methods). Rg-, gG- and g-positive broods represent the G3 broods containing at least one TS3-Rg2, TS3-gG2 and TS3-g2 larva, respectively.
  2. aTotal number of G3 broods containing at least one TS3-Rg2, TS3-gG2 or TS3-g2 larva.
Back to article page