Figure 3
APP is preferentially degraded through the proteasome pathway in response to ER-stress.
(A), CHO cells were pretreated with 10 μM MG132, 1 mM 3MA, 20 mM NH4Cl, or 10 nM Bafilomycin (Baf) for 30 min and later treated with A23187 (1 μM) for 12 h. Representative western blots show APP (22C11) and β-actin expression. (B), Quantification of APP. Statistical significance was tested by one-way ANOVA followed by a Tukey's multiple comparison test (n = 5; *P < 0.05, **P < 0.01). All the gels were run under the same experimental conditions. For each experiment, APP level was quantified by densitometry and normalized to β-actin loading control. Full-length images are presented in the supplementary information.
