Figure 3 | Scientific Reports

Figure 3

From: Amphiregulin activates human hepatic stellate cells and is upregulated in non alcoholic steatohepatitis

Figure 3

Effect of exogenously added AR on hHSC proliferation.

(A) Primary hHSC treated with AR in the picogram range (10 & 100 pgs) are induced to proliferate. Cell division is significantly increased (* p < 0.05) compared to FBS (10%) control and to a known established stellate cell mitogen, platelet derived growth factor (PDGF, 20 ng/ml). (B) Results were confirmed by DAPI staining which enumerated the number of new nuclei in FBS-treated cells or AR (10 pg) + FBS − treated cells. (C) AR treatment (10 pg) of primary hHSC resulted in higher live cell populations as determined by flow cytometry.

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