Figure 4 | Scientific Reports

Figure 4

From: A Lower pH Value Benefits Regeneration of Trichosanthes kirilowii by Somatic Embryogenesis, Involving Rhizoid Tubers (RTBs), a Novel Structure

Figure 4

RTBs and their free-hand slices stained with borax-toluidine blue showing the internal structures and macroscopic morphology of RTBs.

(A–D) RTBs at an early developmental stage. (A) Longitudinal-section slices of RTBs at an early developmental stage. (B) RTB cap. (C) The cortex cells of rhizoids after peeling the exodermis. (D) The conducting tissue of rhizoids after peeling the partial cortex. (E) RTBs formed at an early developmental stage. (F) Enlarged view of E showing an early embryoid rudiment with rapid cell division. (G) Embryoids at a middle-late developmental stage. (H) Embryoids at a middle-late developmental stage (indigo). (I) Longitudinal-section slices of RTBs at a late developmental stage. (J) Embryoids burst exodermis and grow out. (K) Free-hand slices showing one embryoid at a late developmental stage. (L) Non-embryogenic tissue in RTB was stained purple. (M) Embryoid in RTB at a late developmental stage after peeling the exodermis of the RTB. (N) Embryoid in RTB at a late developmental stage after removing about half of the exodermis. (O) Embryoid covered with exodermis in RTB at a late developmental stage. (P) RTB at a late developmental stage with germinating embryoids. Scale bars for (A, E, G, H, I, J, K, L, N, O and P), 0.3 cm. Scale bar for B, 0.15 cm. Scale bars for (C, D, F and M), 100 μm.

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