Figure 3
Human iPSC-derived RPC regenerative potential in experimental AKI.
(a) Human iPSC-derived RPCs identified in mice with cisplatin-induced AKI by human mitochondrial (h-Mito) staining (red) or HNA staining (green). Scale bars: 10 μm. (b) Human iPSC-derived RPCs, pre-labelled with PKH26 cell tracker (red, arrows) identified in cisplatin mice, express AQP1 (white, left panels). Immunoperoxidase staining of renal sections of cisplatin mice receiving saline or iPSC-derived RPCs (serial sections) labelled with h-Mito or AQP1 antibody (central and right panels). In serial sections, the areas encircled by a black line represent the same tubule. Human kidney section is shown as positive control for h-Mito staining. Scale bars: 20 μm. (c) Renal function expressed as blood urea nitrogen (BUN) in mice with cisplatin induced AKI given saline, undifferentiated iPS cells (iPSCs) or iPSC-derived RPCs at 4 days (mean ± SEM, n = 5, *p < 0.01 versus Control; °p < 0.01 vs cisplatin plus saline or iPSCs). (d) Histological evaluation of kidney samples from control, cisplatin mice receiving saline, undifferentiated iPS cells or iPSC-derived RPCs, 4 days after cisplatin (mean ± SEM, n = 5, *p < 0.01 versus Control; °p < 0.01 vs cisplatin plus saline or iPSCs). Scale bars: 50 μm.
