Figure 2
Antibacterial activities of decomposed vitamin D3 species against H. pylori.
(a) Three vitamin D3 species dispersed into distilled water were incubated for 24 to 48 h at 70°C, recovered via the organic solvent distribution method and analyzed by TLC. D3, vitamin D3; 25D3, 25-hydroxyvitamin D3; 1,25D3, 1α,25-dihydroxyvitamin D3. (b) The decomposition products obtained from the 48-h incubation of the experiments described in panel (a) were incubated for 2 h in the bacterial suspensions of FC-free H. pylori and the CFUs were then counted. The minus and plus symbols in the graph are the CFU levels of H. pylori incubated in the presence of the intact D3 species and decomposed D3 species, respectively. The control in the graph is the CFU level of H. pylori incubated without any D3 specimens. The dashed line bar in the graph represents the baseline CFU measured immediately after the incubation was started. Three independent experiments with the respective D3 species were performed to calculate the mean CFU ± SD per ml.
