Figure 4
Anti-H. pylori action of the purified VDP1 and VDP2 and interaction of the VDPs with the PE vesicles.
(a) FC-free H. pylori or FC-retaining H. pylori was incubated for 24 h in the presence of either VDP1 or VDP2 in a broth with or without FC (30 μM) and the CFUs were counted. The gray bars in the graphs represent the baseline CFU counts. The mean CFU ± SD per ml was obtained from three independent experiments. (b) PE vesicle suspensions containing CBB were shaken for 2 h in the presence of either VDP1 or VDP2 and A590 nm was measured in the supernatant. CBB elution was denoted by the relative A590 nm to the A590 nm measured as a value of 1 in the supernatant of the PE vesicles incubated without VDPs. The statistical significance of differences between the CBB elutions from the DMPE vesicles and DPPE vesicles was evaluated by the t-test based on data obtained from three independent pair-experiments. N.S. stands for “no statistical significance”.
