Figure 4
From: Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection
(A) Fluorescence emission spectra of FRET were recorded for 0–5 nM sarcosine at 25°C. The excitation wavelength was set to 400 nm. In inset: there is the expression of fluorescence (λexc 400 nm) dependence on sarcosine concentration evaluated in both emission maxima of GFP (λem 510 nm) and QDs (λem 604 nm). (B) Saturation curve of sarcosine (0–500 nM, diluted in PBS, pH 7.4). In inset: the calibration curve determined within linear range 5–50 nM. (C) The expression of interfering potential of various amino acids (all of them diluted in PBS, pH 7.4 to the final 50 nM concentrations) on FRET efficiency. Values are means of three independent replicates (n = 3). Vertical bars indicate standard error.
