Figure 1
From: Human iPSC-based Cardiac Microphysiological System For Drug Screening Applications
The cardiac microphysiological system (MPS).
(a) Schematic of the MPS nutrient channels (red) and cell-loading channel (green). (b) Scanning electron micrograph of the MPS showing in inset the 2 μm endothelial-like barriers connecting the nutrient channel and the cell channel. Red rectangular box shows the weir that enables efficient and consistent loading of singularized high density hiPSC-CMs. (c) Schematic of the cardiac MPS showing nutrient inlet and outlet ports connected to tubes. (d) Simulated velocity profile of flow in the MPS, inset shows the magnified view. Note the lack of convection within the diffusive barriers and predominant convective flow through the nutrient channels. Thus, mass transport to the tissue is exclusively diffusive. (e) Diffusion dynamics in the microphysiological system. Normalized fluorescence recovery of 4 kDa FITC–dextran (0.2 mg/mL). Insets are confocal microscopy images corresponding to the FRAP experiment. Finitial is the time regime that corresponds to the initial fluorescence before bleaching; F0 is the fluorescence measurement immediately after photobleaching; Ft > 0 corresponds to the recovery of fluorescence after photobleaching; Ft ≫ 0 corresponds to maximal recovery of fluorescence at the end of the experiment. Scale bar: 10 μm.
