Figure 2
From: Testosterone regulates the autophagic clearance of androgen binding protein in rat Sertoli cells
ABP colocalises with LC3 in primary Sertoli cells.
(A), Rat primary Sertoli cells were treated with CQ (50 uM) or rapamycin (10 nM) for 24 h (n = 3), or treated with ATG7 or MTOR siRNA for 48 h (n = 3), ABP mRNA was determined by qPCR. (B–C), Cells were treated with CQ or rapamycin for 24 h and ABP and LC3 were assessed with double immunofluorescence (B). The average number of colocalisations per cell was calculated from 10 random fields (approximately 200 cells) (C) (n = 3, **p < 0.01).
