Table 1 Concentrations of cytokines and chemokines produced by ESDM: A. WT ESDM: After 4 h stimulation with various TLR agonists, or at various times during infection with S. Typhimurium SL1344 (p1C/1, ssaG::GFP). B. Comparison of WT, traf2^−/+ and traf2^-/- ESDM. Results were obtained after 24 h stimulation with TNFα, or 4 h stimulation with LPS or flagellin. Each value in parts A and B represents the average of at least 3 independent measurements ± standard deviation. Equal numbers of WT and mutant cells were seeded for the various stimulation experiments

			Cytokines and Chemokines Induction (pg/ml)
Stimulus	Time	Cell Type	IP10	TNFα	KC	MIP1α	MIP1β
A.
LPS	4 h	WT	1201 ± 460	620 ± 196	1562 ± 389	5133 ± 602	8445 ± 808
Flagellin	4 h	WT	656 ± 244	607 ± 180	1731 ± 264	3915 ± 809	6221 ± 714
CpG ODN	4 h	WT	725 ± 312	198 ± 28	321 ± 124	1097 ± 567	5934 ± 670
Poly-IC	4 h	WT	1389 ± 431	43 ± 13	100 ± 15	1038 ± 218	1915 ± 105
Salmonella	1 h	WT	26 ± 6	76 ± 13	241 ± 55	69 ± 19	71 ± 22
Salmonella	2 h	WT	119 ± 54	160 ± 52	719 ± 69	263 ± 89	381 ± 76
Salmonella	4 h	WT	893 ± 28	487 ± 70	1996 ± 231	1416 ± 110	2342 ± 332
Salmonella	6 h	WT	1373 ± 110	735 ± 89	2883 ± 452	3203 ± 219	7832 ± 987
B.
LPS	4 h	WT	1055 ± 224	619 ± 248	1547 ± 619	4907 ± 1472	8052 ± 1240
		traf2 ^−/+	756 ± 213	550 ± 102	2122 ± 1412	3395 ± 1133	7639 ± 890
		traf2 ^-/-	582 ± 131*	155 ± 78*	4771 ± 1400*	1694 ± 900*	4089 ± 524*
Flagellin	4 h	WT	701 ± 79	665 ± 120	2017 ± 334	4113 ± 1808	7390 ± 2450
		traf2 ^−/+	449 ± 124	758 ± 113	2921 ± 400*	2576 ± 942	4521 ± 1156
		traf2 ^-/-	64 ± 24*	303 ± 68*	3116 ± 399*	773 ± 831*	1031 ± 1000*
TNFα	4 h	WT	5008 ± 725	3104 ± 519	-	-	-
		traf2 ^−/+	5615 ± 790	3222 ± 600	-	-	-
		traf2 ^-/-	7596 ± 890*	5637 ± 714*	-	-	-

Values are shown as net cytokine/chemokine production, which was calculated by subtracting the value for the unstimulated control. Each value represents the average of at least 3 measurements ± standard deviation. "-" means not tested *Indicates a significant difference (P < 0.05) between mutant and WT.

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