Figure 6 | Scientific Reports

Figure 6

From: Systematic identification and characterization of long intergenic non-coding RNAs in fetal porcine skeletal muscle development

Figure 6

Features of the ENSMUSG00000090086 gene.

(A) Q-PCR analysis of ENSMUSG00000090086 gene expression during C2C12 cell culture in growth medium (GM) or differentiation medium (DM) for 1, 3 and 5 days (here and below, the values represent the means ± s.e.m., n = 6). (B) RT-PCR analysis of Myog and MHC expression to monitor the differentiation status at indicated times. HPRT was used as an endogenous control. (C) RT-PCR analysis of the relative expression of iso1 and iso4 as well as other control genes in the nuclear and cytoplasmic cell fractions. (D) The RIP result of iso1, iso4 and HOTAIR with the EZH2 antibody. RIP enrichment was measured by q–PCR and the values were normalized to background levels and input samples (Percent Input Method). The interaction of HOTAIR with EZH2 is a known interaction that served as a positive control(**indicates P < 0.01, here and below, the values represent the means ± s.e.m., n = 3).

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