Figure 3

ADP-ribosylation inhibits binding of anti-CD25 mAb 7D4 but not of mAb PC61.

Splenocytes from DEREG mice were incubated in the absence or presence of the indicated concentrations of NAD⁺ before staining with fluorochrome-conjugated mAbs directed against CD4 and CD25 (mAb PC61 or mAb 7D4). Gating was performed on CD4⁺ cells. (a) Representative dot plots of cells incubated in the absence or presence of 12 μM NAD⁺. (b) Percentages of CD4⁺GFP⁺ cells staining with anti-CD25 mAbs plotted as a function of the concentration of added NAD⁺. Results are representative of two independent experiments.