Figure 4
From: Prevention of exercised induced cardiomyopathy following Pip-PMO treatment in dystrophic mdx mice
Reduction in cardiac pathology as determined by the detection of fibrosis and sarcolemmal damage in the hearts of exercised mdx mice following Pip6f-PMO treatment.
(A) RT-qPCR analysis of miRNA-21 in heart tissue normalised to C57BL/10 cohort. (B) Quantification of Masson's trichrome staining in hearts of exercised cohorts. (C) Evans blue dye infiltration into heart following exercise. (D) Masson's trichrome images of worst areas of collagen deposition. miRNA-21 expression and Evans blue dye leakage of Pip6f-PMO hearts is normalised in contrast to untreated control. In addition Masson's trichrome staining is reduced and the representative images indicate less fibrosis then the untreated mdx cohort. Statistical significance was determined using ANOVA followed by Tukey post-hoc test (*** = P < 0.001, ** = P < 0.01, * = P < 0.05).
