Figure 5 | Scientific Reports

Figure 5

From: Bilberry extract (Antho 50) selectively induces redox-sensitive caspase 3-related apoptosis in chronic lymphocytic leukemia cells by targeting the Bcl-2/Bad pathway

Figure 5

Antho 50 decreases cell viability and triggers apoptosis in B CLL cells through generation of ROS.

Cells were exposed to Antho 50 (75 μg/mL) for different times (A) or to various inhibitors of ROS (B) for 30 min before the addition of Antho 50 (75 μg/mL) for 1 h. The formation of ROS was assessed by flow cytometry after incubation with the redox-sensitive fluorescent probe DHE. Cells were incubated with various inhibitors of ROS for 30 min before the addition of Antho 50 (75 μg/mL) for 6 h for cell viability analysis (C) or for 24 h before the determination of apoptosis (D). The control (Ctr) represents untreated cells harvested at 6 h. The data are representative of cells from three CLL patients for ROS analysis, three CLL patients for cell viability and four CLL patients for apoptosis analysis.

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