Figure 6
From: New Anti-angiogenic Leading Structure Discovered in the Fruit of Cimicifuga yunnanensis
In vitro and ex vivo anti-angiogenic activities of compound 1.
(A) The proliferation inhibitory effect of 1 against normal and VEGF-induced HUVECs; (B) Compound 1 inhibited the VEGF-induced migration of HUVECs. Cells were wounded with a pipette then treated with vehicle or 2.5 μM sunitinib and compound 1. After 6, 12 and 24 h, the migrated cells were quantified by the Image Pro Plus software; (C) Compound 1 inhibited ex vivo angiogenesis in CAM assay, quantification of the number of new vascular growth counts was performed with Image Pro Plus software. *p < 0.05, **p < 0.01, ***p < 0.001 vs vehicle control, data were analyzed by using Graphpad student t test (n = 3).
