Figure 7
From: Loss of muscleblind-like 1 results in cardiac pathology and persistence of embryonic splice isoforms
Mbnl1 depletion results in the persistence of embryonic splice isoforms in adult hearts.
(a). A cardiac RNA network that is misspliced in Mbnl1ΔE2/ΔE2 hearts is shown. RNAs are categorized by their biological function and Mesh terms. (b–g). Splicing analysis for the indicated RNAs was performed by RT-PCR with E18 Mbnl1+/+ and 4 months old male Mbnl1+/+ (n = 3) and male Mbnl1ΔE2/ΔE2 (n = 3) hearts. Band intensities were quantified by densitometry. Data are standard error of mean (SEM). p-values were calculated using Student's t-test with significance set at p ≤ 0.05. Primer locations, exon numbers and expected band sizes are indicated. The alternatively spliced exons are shown as green boxes. Exon numbers for all genes except Junctate/Asph are annotated based on Refseq from the UCSC genome browser (NCBI37/mm9); Junctate/Asph is annotated based on the study carried out by Dinchuck et al (Dinchuk et al., 2000). Scn5a exon 6 and 6A were distinguished by digestion with SacI. The SacI site within Scn5 exon 6A is indicated in red. Identified novel exons are indicated by asterisks and the sequences of these exons are shown in Supplementary figure S9. The images shown are cropped. The full-length gels are shown in Supplementary figures S10–S15.
