Figure 4
Effect of 2-Cl-IB-MECA on DSS-induced NF-κB p65 signaling pathway activation in murine colon epithelia.
Cytosolic and nuclear proteins were extracted separately and analyzed by western blot. DSS administration resulted in a reduction in IκB-α (A, B) and an increase in p-IκB-α (A,C) in the cytoplasm and an increase of NF-κB p65 (A,D) in nuclear extracts from murine colon epithelia, indicating DSS colitis induced phosphorylation and degradation of IκB-α in the cytoplasm and NF-κB p65 translocation from the cytoplasm to the nucleus. Compared with the DSS colitis group, 2-Cl-IB-MECA treatment attenuated the expression of p-IκB-α in the cytoplasm and decreased the expression of NF-κB p65 in the nucleus, suggesting 2-CL-IB-MECA inhibited DSS colitis and induced phosphorylation of IκB-α in the cytoplasm and NF-κB p65 nuclear translocation. (*P < 0.01 compared with the normal group; #P < 0.05 compared with the DSS group and DMSO group). The blots in panel A were performed under the same experimental condition except for blotting with the different antibodies, respectively. These blots were shown as cropped images.
