Figure 3 | Scientific Reports

Figure 3

From: Magnetic Resonance Imaging with Superparamagnetic Iron Oxide Fails to Track the Long-term Fate of Mesenchymal Stem Cells Transplanted into Heart

Figure 3

(A&B) Morphology of the MSCs under the inverted microscope. (A) The phenotype of the cells at passage 0 was heterogeneous (round shaped, irregular shaped and spindle shaped cells) forming different cell colonies. (original magnification, 100×). (B) The phenotype of the passage 3 cells was homogeneous spindle shaped cells at 80–90% confluence (original magnification, 40×). (C) Eosin and Prussian blue staining of SPIO-labeled MSCs. The labeling efficiency of MSCs using SPIO and PLL reached 90%. Clustered blue particles were present in most of the cells (original magnification, 100×). (D) DAPI-labeled MSCs under the inverted fluorescence microscope. DAPI-labeled MSCs at passage 3 observed by fluorescence microscopy (original magnification, 100×).

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