Figure 3 | Scientific Reports

Figure 3

From: Structure, Function and Inhibition of the Phosphoethanolamine Methyltransferases of the Human Malaria Parasites Plasmodium vivax and Plasmodium knowlesi

Figure 3

Amodiaquine (AQ) and NSC158011 inhibit the activity of PvPMT and PkPMT.

Enzyme inhibition assays with different concentrations of Amodiaquine (AQ), NSC158011 and Chloroquine (CQ) were carried out at constant P-Etn (2 mM) and SAM (1.5 mM) concentrations. The activity in the absence of any drug was taken as 100% and % enzyme activity was calculated in the presence of different drug concentrations (100, 500, 1000 μM). To determine the concentration that results in 50% inhibition of enzyme activity (IC50), the data were fitted to a non-linear regression analysis in the following equation Y = 100/(1 + ([X]/IC50)), where Y is % enzyme activity and X is drug concentration using Graph pad software.

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