Figure 4 | Scientific Reports

Figure 4

From: PERK silence inhibits glioma cell growth under low glucose stress by blockage of p-AKT and subsequent HK2's mitochondria translocation

Figure 4

PERK silencing decreased p-AKT in glioma cells under low glucose metabolism stress.

U87 and U251 cells were transfected with NC or PERK shRNA and then were treated by 2-DG, BRPA or grown in LGM for 48 h. Westernblot analyses were performed to detect p-AKT (Ser473), AKT, PERK p-PERK, p-EIF2α and EIF2α protein expression. Statistical analysis of p-AKT/AKT was derived from 3 independent experiments which were run under same conditions. *, P < 0.05, **, P < 0.01.

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