Figure 5

EGF rescued p-AKT level in PERK silenced glioma cells under low glucose metabolism stress.
U87 and U251 cells were transfected with NC or PERK shRNA, then 48 hours later the cells were treated under LGM or incubated by 2-DG or BRPA for 48 hours with or without pretreatment of EGF for 6 hours. (a) Westernblot analyses were performed to detect p-AKT protein expression. (b) Statistical analysis of p-AKT/AKT was derived from 3 independent experiments which were run under same conditions. **, P < 0.01.