Figure 6 | Scientific Reports

Figure 6

From: PERK silence inhibits glioma cell growth under low glucose stress by blockage of p-AKT and subsequent HK2's mitochondria translocation

Figure 6

EGF rescued cell viability in PERK silenced glioma cells under low glucose metabolism stress.

U87 and U251 cells were transfected with NC or PERK shRNA, then 48 hours later the cells were treated under LGM or incubated by 2-DG or BRPA for 48 hours with or without pretreatment of EGF for 6 hours. (a–b) Cell viability was analyzed by MTT. (c–d) ATP production was examined by ATP kit. (e–f) Lactate production was examined by lactate kit. The results repeated three times and the data shown represent mean ± SEM of three independent experiments. *, P < 0.05.

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