Figure 7
SchB treatment induces transient lipolysis in fasting mice.
Relative free fatty acid (FFA) levels in (A) fasting mice, (B) non-fasting mice and (C) HFD-fed mice. Shown is the mean ± SE (n = 10 mice in each group), **p < 0.01. Representative western blotting showing the expressions of adipose triglyceride lipase (ATGL), hormone sensitive lipase (HSL), phospho-HSL (Ser-563) in adipocytes isolated from the bilateral superficial subcutaneous adipose tissue (SA), prominent bilateral intra-abdominal visceral depots attached to the epididymides (EA) and the perirenal fat (RA) (D–E) 24 h after fasting, (F) 2 h after fasting, (G) 6 h after fasting and (H) 12 h after fasting. Full length blots were shown in Supplementary S8. (I) The basal and isoproterenol (10 μM) stimulated fatty acids (FAs) released from subcutaneous adipose tissue dissected from fasting mice 2, 6 and 12 h after SchB treatment. Shown is the mean ± SE (n = 3 independent experiments), *p < 0.05. a < 0.05 compared to basal nf-veh. b < 0.05 compared to stimulated nf-veh. nf-veh: non-fasting vehicle control group; nf-SchB: non-fasting SchB-treated group; f-veh: fasting vehicle control group; f-SchB: fasting SchB-treated group; HFD-veh: HFD-fed vehicle control group; HFD-SchB: HFD-fed SchB-treated group.
