Table 1 Oligonucleotides used for measurement of the binding strength of dA/dT and dG/dC base paris

From: Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope

 

sequences

notation

SH1

5′-SH-(CH2)6-(G)30-3′

Cantilever tip

SH2

3′-SH-(CH2)6-(G)30-5′

Cantilever tip

dC10

3′-NH2-(CH2)6-(C)10-5′

Glass slide

dC14

3′-NH2-(CH2)6-(C)14-5′

Glass slide

dC20

3′-NH2-(CH2)6-(C)20-5′

Glass slide

SH3

5′-SH-(CH2)6-(A)30-3′

Cantilever tip

SH4

3′-SH-(CH2)6-(A)30-5′

Cantilever tip

dT10

3′-NH2-(CH2)6-(T)10-5′

Glass slide

dT14

3′-NH2-(CH2)6-(T)14-5′

Glass slide

dT20

3′-NH2-(CH2)6-(T)20-5′

Glass slide