Table 4 Binding strength obtained using the unzipping mode for dG/dC base pair of different lengths

From: Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope

Length of nucleotide chain(bp)

Hydrogen bond force (nN) Cluster centers distribution (%)

10

0.2029 (32.72)

0.3990 (28.87)

0.6056 (5.80)

0.8041 (13.49)

1.0031 (11.57)

1.2293 (7.73)

14

0.2837 (41.96)

0.5441 (27.06)

0.8442 (9.81)

1.1519 (10.98)

1.4467 (6.67)

2.0958 (3.53)

20

0.4019 (37.52)

0.8227 (28.95)

1.2124 (17.78)

1.5794 (8.95)

2.0370 (3.72)

2.4636 (3.14)

  1. Data were obtained under condition of 100 mM NaCl concentration and 10 s holding time. Data in parentheses are the percentage of the binding strengths in the whole clustering data.
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