Figure 1 | Scientific Reports

Figure 1

From: Mitochondrial superoxide in osteocytes perturbs canalicular networks in the setting of age-related osteoporosis

Figure 1

Superoxide generation is upregulated in osteocytes during aging.

(a) Young (8 weeks of age) and aged (2 years of age) femurs were stained with dihydroethidium (DHE) (n = 3 each group). The scale bar indicates 20 μm. (b) AgNOR staining of osteocytic canaliculi in the endosteal region of the young (12 weeks of age) and aged (2 years of age) cortical femurs. The number of osteocyte canaliculi (N.Ot.Ca.) per osteocyte lacuna (Ot.Lc.) in the cortical femurs. The scale bars indicate 50 and 10 μm (inset), respectively. (c, d) Decalcified femoral sections were prepared from young (12 weeks of age) and aged (2 years of age) femurs and subjected to sclerostin and RANKL immunohistochemical staining. The scale bars indicate 50 μm. (e) The gene expression analysis of osteocyte-related genes in the flushed tibia obtained from young (12 weeks of age) and aged (2 years of age) mice. The data were normalized to the 18s expression (n = 3–4 each group).

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