Figure 1 | Scientific Reports

Figure 1

From: The effects of Rho-associated kinase inhibitor Y-27632 on primary human corneal endothelial cells propagated using a dual media approach

Figure 1

Culture and characterization of primary human CECs.

(A) Schematic diagram depicting the expansion strategy of human CECs using a dual media approach, inter-switching between a M5-Endo medium for cellular stabilization/maintenance and a M4-F99 medium for cell proliferation. (B) Representative micrograph of a confluent homogeneous monolayer of P0 human CECs maintained for several days in M5-Endo medium following proliferative expansion in M4-F99 medium. Representative micrographic indirect immuno-fluorescent images of human CECs taken at the first passage which express (C) NA+/K+-ATPase, (D) ZO-1, (E) GPC-4 and (F) CD-200, indicative of the corneal endothelium. Scale Bars – B: 100 μm; C–F: 50 μm.

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