Figure 3

Exposure to Y-27632 increases the proliferation rates of primary human CECs.
(A) Click-iT EdU assay was carried out to assess the cell proliferation of human CECs in M4-F99 medium with or without addition of 10 μM Y-27632 over a period of 24 hours (n = 5; *p < 0.05). (B) Representative flow cytometric dot plots of BrdU incorporation of human CECs grown in M4-F99 medium with or without exposure to 10 μM Y-27632 over a period of 48 hours (n = 4; *p < 0.05). Scale Bars: 100 μm.