Figure 1

SlDMT silencing causes Cnr epimutant to ripening.
(a), Context, number and percentage of the hypermethylated cytosines (5mC) in the 286-bp LeSPL-CNR promoter region. (b), Diagram of VIGS vectors PVX/SlDRM7, PVX/SlMET1, PVX/SlCMT2, PVX/SlCMT3, PVX/SlCMT3UTR and PVX/SlCMT4. (c–h), Ripening in Cnr fruits, assessed by red colour as compared to wild-type fruits (AC, (e)). No ripening was observed in fruits mock-inoculated (mock), inoculated with PVX or PVX/SlCMT4 (h). Photographs were taken at the indicated day post-anthesis (dpa). Bar = 1 cm. (i), Number of ripening fruits out of total number of inoculated fruits from at least two independent experiments.